Isolation and speciation

Energy Flow, Ecosystems and the Environment · Temperature, evolution and speciation · note 4 of 4

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Isolation and speciationSpec 5.24

In short

A species is a group of organisms that can interbreed to produce fertile offspring. Isolation reduces gene flow between populations, so different mutations and selection pressures cause their allele frequencies to diverge. If they can no longer interbreed, new species form: allopatric speciation after geographical isolation, or sympatric speciation within the same area.

A species is a group of organisms that can interbreed to produce fertile offspring. Gene flow is the movement of alleles between populations by interbreeding. Speciation is the formation of new species. It requires that gene flow between populations is reduced or stopped by isolation.

Allopatric speciation

  1. A population is divided by a geographical barrier such as a mountain range, river, sea or desert. The two populations can no longer interbreed, so there is no gene flow between them.
  2. Mutations occur independently in each population, and the selection pressures differ between the two environments.
  3. Allele frequencies in the two gene pools gradually diverge.
  4. Eventually the populations are so different (for example in courtship behaviour, breeding time, or gamete compatibility) that if they came into contact again they could not interbreed to produce fertile offspring. They are now separate species.

Sympatric speciation

In sympatric speciation, new species form within the same geographical area, without a physical barrier. Reproductive isolation arises in other ways:

  • Ecological or habitat isolation: groups use different parts of the habitat or different food sources.
  • Temporal isolation: groups breed at different times of the year or day.
  • Behavioural isolation: changes in courtship behaviour mean that individuals do not recognise each other as mates.
  • Polyploidy (common in plants): an error in cell division (for example meiosis that produces gametes with two sets of chromosomes) doubles the number of chromosome sets. The polyploid plants cannot produce fertile offspring with the parent species (a cross gives sterile offspring), but they can reproduce with each other or by self-pollination.
Allopatric and sympatric speciation
FeatureAllopatricSympatric
LocationPopulations in different areasPopulations in the same area
Cause of isolationGeographical barrierEcological, temporal, behavioural or genetic changes
Gene flowStopped by the barrierReduced by reproductive isolation
OutcomeDifferent allele frequencies, new speciesDifferent allele frequencies, new species
Two routes to speciation: in allopatric speciation a barrier separates populations that diverge under different selection pressures until they cannot interbreed, and in sympatric speciation groups in the same area stop interbreeding because of different habitats or breeding times. (opens full size in a new tab)
Allopatric and sympatric speciation.
Exam tip:

Always include the same logic: isolation, so no gene flow, so different mutations and selection, so allele frequencies diverge, so no interbreeding (reproductive isolation), so new species.

Quick check

  1. Write the formula for Q₁₀.

    Show answer

    Rate at (T + 10) °C ÷ rate at T °C.

  2. Why does the rate of an enzyme-controlled reaction fall above the optimum temperature?

    Show answer

    The enzyme denatures: bonds break and the active site changes shape.

  3. Define evolution.

    Show answer

    A change in allele frequency in a population over generations.

  4. What is the difference between allopatric and sympatric speciation?

    Show answer

    Allopatric involves geographical isolation. Sympatric occurs in the same area.

Written and checked against the Edexcel International A Level Biology (YBI11) specification · Updated October 2026

Frequently asked questions

What does Q10 mean in biology?

Q₁₀ is the temperature coefficient: the factor by which the rate of a reaction increases for a 10 °C rise. It is found by dividing the rate at the higher temperature by the rate at the lower temperature. For enzyme-controlled reactions it is often about 2 below the optimum.

Why do enzymes denature at high temperatures?

High temperature gives enzyme molecules so much kinetic energy that hydrogen and other bonds holding the tertiary structure break. The active site changes shape, and the substrate can no longer fit. Denaturation is usually irreversible, so the rate of reaction falls.

How does natural selection lead to evolution?

Mutations create new alleles. If an allele gives an advantage, its carriers are more likely to survive and reproduce, passing the allele on. Over generations the frequency of the allele increases in the population, and this change in allele frequency is evolution.

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