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Water potential

Movement into and out of cells · Osmosis · note 3 of 4

Spec 3.2.7, 3.2.9
Supplement (what this means)Supplement: only for the Extended papers (2 and 4). Core students can skip it. What the labels mean
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Water potentialSpec 3.2.7, 3.2.9

In short

Osmosis is the net movement of water molecules from a region of higher water potential (dilute solution) to a region of lower water potential (concentrated solution), through a partially permeable membrane. Pure water has the highest water potential. Root hair cells have a lower water potential than the soil water, so water enters them by osmosis.

Water potential describes how freely water molecules can move. A dilute solution has a high water potential. A concentrated solution has a low water potential. Pure water has the highest water potential.

Osmosis
The net movement of water molecules from a region of higher water potential (dilute solution) to a region of lower water potential (concentrated solution), through a partially permeable membrane.

When a dilute sugar solution and a concentrated sugar solution are separated by a partially permeable membrane, the dilute solution has the higher water potential and the concentrated solution has the lower water potential. Water moves through the partially permeable membrane down this water potential gradient.

Exam tip:

Use the phrase 'water potential' and the word 'net'. Say 'from a higher water potential to a lower water potential', not 'from a weak solution to a strong solution' alone.

Importance in organisms

  • Plants: root hair cells have a lower water potential than the soil water, so water enters them by osmosis. The water then moves from cell to cell along a water potential gradient into the xylem.
  • Plants: loss of water from leaf cells lowers their water potential, so water is drawn in from neighbouring cells.
  • Animals: if the blood has a lower water potential than the cells, water leaves the cells. If the blood has a higher water potential, water enters the cells. Animal cells have no cell wall, so they can burst or shrink.
  • Organisms must keep the water potential of their body fluids in a narrow range so that cells neither swell too much nor lose too much water.
Common mistake:

Do not describe water potential as the 'amount of water'. Explain what happens by comparing the water potential of the solution with that of the cell.

Written and checked against the Cambridge IGCSE Biology (0610) specification · Updated October 2026

Frequently asked questions

How does osmosis differ from diffusion?

Osmosis is a type of diffusion that involves only water moving through a partially permeable membrane. Diffusion is the net movement of any particles, such as oxygen or carbon dioxide, down a concentration gradient. Both are passive, so neither needs energy from respiration. Never write that sugar moves by osmosis.

How does osmosis affect plant cells?

Plant cells in a dilute solution take in water by osmosis, and the water presses outwards on the cell wall, making the cells firm. Firm cells support the stems and leaves of non-woody plants. In a concentrated solution, plant cells lose water, the pressure falls, the cells become soft and the plant wilts.

Why don't plant cells burst in pure water?

Supplement Plant cells do not burst in pure water because the strong cell wall resists the pressure. Water enters by osmosis, the vacuole swells and presses the cell contents against the wall, and the cell becomes turgid. Animal cells have no cell wall, so if they take in too much water they can burst.

All 5 questions on Osmosis