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Practical skills — Cambridge IGCSE Biology

Planning, tables, graphs, biological drawings, magnification, evaluating experiments, indicators and random sampling for Papers 5 and 6.

Spec S1–S7Experimental skills, subtopic 1 of 1

Revision notes

7 short notes, in the order of the specification. Each one in short:

  1. Planning an investigation means stating exactly what you change, what you measure, what you keep the same and how. The independent variable is changed, usually over at least five evenly spaced values; the dependent variable is measured with named apparatus. Keep controlled variables constant, include a control experiment, repeat each test at least three times and calculate a mean.

  2. A Cambridge results table is ruled, with the independent variable in the first column and each heading giving the quantity and unit separated by a solidus, such as time / s. Units go only in the heading, never in the body. Record data to the precision of the instrument, to the same decimal places in each column.

  3. A line graph is used when the independent variable is continuous, such as temperature, while a bar chart, with bars that do not touch, is used for categories. Put the independent variable on the x-axis, label axes with quantity and unit, use more than half the grid, plot small crosses and draw a single thin best-fit line or curve.

  4. A biological drawing must be large, with clear, unbroken pencil lines, no shading or colour, and ruled label lines that touch the feature without arrowheads. Measure sizes in millimetres with a ruler. Magnification equals image size divided by actual size, both in the same unit, and has no unit; it is written with a × sign, such as ×3.

  5. Evaluating an experiment means judging how good the method and data are, then suggesting specific improvements. Identify any anomalous result, a result that does not fit the pattern, and leave it out of the mean. Name sources of error and exact fixes, such as using a syringe instead of a beaker. Human error or being more accurate scores nothing.

  6. Indicators are substances that change colour to show a chemical change, and you must give the colour before and after. Limewater turns from colourless to milky with carbon dioxide. Hydrogencarbonate indicator is red at the level in air, yellow when carbon dioxide increases and purple when it decreases. Universal indicator gives the pH number; litmus shows only acid or alkali.

  7. Random sampling is choosing samples so that every individual or position has an equal chance of being chosen, for example using random number coordinates on a grid of tape measures. It avoids bias, which can happen when you choose positions yourself or throw a quadrat. A larger sample size, such as 10 to 20 quadrats, makes results more representative.

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Quick check questions

  1. How should a column heading for time in seconds be written?

    Show answer

    time / s

  2. Which variable goes on the x-axis?

    Show answer

    The independent variable, unless told otherwise.

  3. State the formula for magnification.

    Show answer

    magnification = image size ÷ actual size

  4. What colour does hydrogencarbonate indicator turn when carbon dioxide increases?

    Show answer

    Yellow (from red).

  5. Why should quadrats be placed at random?

    Show answer

    To avoid bias.

Frequently asked questions

How do you calculate magnification in biology?

Magnification is calculated as image size divided by actual size. Measure the image, such as your drawing or a photograph, with a ruler in millimetres, and make sure both sizes are in the same unit. For example, a leaf 45 mm long drawn 135 mm long has a magnification of ×3. Magnification has no unit.

What is the difference between independent and dependent variables?

The independent variable is the one the scientist changes, and the dependent variable is the one observed or measured. Changing the independent variable may cause a change in the dependent variable. For example, in an enzyme experiment you change the temperature and measure the time taken for the starch to disappear. Other variables are controlled.

How do you draw a results table in biology?

Draw a ruled table with a border and lines between columns. Put the independent variable in the first column and the dependent variable, with repeats and the mean, in the next columns. Head each column with the quantity and unit separated by a solidus, such as time / s, and keep units out of the body.

What is an anomalous result?

An anomalous result is a result that does not fit the pattern of the other results. Identify it by comparing it with the repeats and the trend. Repeat that test if you can, leave it out when you calculate the mean, and ignore it when you draw a best-fit line. Repeats make anomalies easier to spot.

How do you use a quadrat for random sampling?

Lay two tape measures at right angles along two edges of the area to make a grid. Use a random number generator to pick two coordinates, place the quadrat there and count the chosen species inside it. Repeat for many quadrats, for example 10 to 20, and calculate the mean number per quadrat.

Written and checked against the Cambridge IGCSE Biology (0610) specification · Updated October 2026