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Rate calculations for enzyme activity

Key concepts in biology · Enzyme action and activity · note 5 of 5

Rate calculations for enzyme activitySpec 1.11

The rate of reaction tells you how fast a reaction is happening. It is a compound measure, because it combines an amount with a time.

rate = amount of product formed ÷ time taken

The units depend on what you measure, for example cm³/s for a volume of gas, or g/min for a mass. If an experiment measures the time taken for a reaction to finish, such as the time for starch to disappear, the rate can be found as 1 ÷ time, with units of s⁻¹.

Calculating a rate

An enzyme produces 12 cm³ of gas in 40 seconds. Calculate the rate of reaction in cm³/s.

  1. rate = amount of product ÷ time
  2. 12 ÷ 40 = 0.3

Answer: 0.3 cm³/s

Quick check

  1. What is the name of the part of an enzyme that the substrate fits into?

    Show answer

    The active site.

  2. Why does an enzyme stop working at a high temperature?

    Show answer

    The shape of the active site changes, so the substrate no longer fits. The enzyme is denatured.

  3. Why does the rate level off as substrate concentration increases?

    Show answer

    All the active sites are occupied.

  4. In the pH core practical, what colour shows that starch is still present with iodine solution?

    Show answer

    Blue-black.

  5. Calculate the rate when 30 cm³ of gas is collected in 60 s.

    Show answer

    0.5 cm³/s.

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