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Estimating the number of organisms

Ecosystems and material cycles · Fieldwork and sampling · note 2 of 2

Estimating the number of organismsSpec 9.6

You cannot count every organism in a large area, so you take samples and use them to estimate the total. A larger number of quadrats, placed randomly, gives a more reliable estimate.

estimated population = mean number per quadrat × (total area ÷ area of one quadrat)

Estimating a population

A student counted daisies in five 0.25 m² quadrats placed randomly in a field of area 100 m². The counts were 3, 5, 4, 6 and 2. Estimate the number of daisies in the field.

  1. Mean per quadrat = (3 + 5 + 4 + 6 + 2) ÷ 5 = 20 ÷ 5 = 4
  2. Number of quadrat areas in the field = 100 ÷ 0.25 = 400
  3. Estimated population = 4 × 400 = 1600

Answer: 1600 daisies

Plants that are difficult to count individually, such as grass, can be measured by percentage cover: the percentage of the quadrat covered by the species.

Other points to understand about sampling:

  • Take as many quadrats as possible, because a bigger sample is more representative and reduces the effect of anomalies.
  • Place quadrats randomly, to avoid bias.
  • Calculate a mean from the raw data. A bar chart or frequency table is useful for presenting the results.

Quick check

  1. How do you stop bias when placing quadrats?

    Show answer

    Use random numbers to choose the coordinates.

  2. What does a belt transect show?

    Show answer

    How the distribution of organisms changes along an environmental gradient.

  3. Write the equation for estimating a population from quadrat data.

    Show answer

    Mean per quadrat × (total area ÷ area of one quadrat).

  4. Why use many quadrats?

    Show answer

    To give a more representative and reliable sample, and reduce the effect of anomalies.

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