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Exchange and transport in animals, subtopic 5 of 5Spec 8.9–8.11

Respiration

Aerobic and anaerobic respiration, how they compare, and the core practical to investigate the rate of respiration in living organisms.

3 sections, with a quick check at the end.

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Cellular respirationSpec 8.9

Cellular respiration is an exothermic reaction. It transfers energy to the surroundings, and some of this energy is released as heat. It happens continuously in living cells (in plants as well as animals) to release energy for metabolic processes.

Metabolic processes include building larger molecules from smaller ones, muscle contraction, and keeping the body warm in mammals and birds.

Aerobic respiration

Aerobic respiration uses oxygen to break down glucose.

glucose + oxygen → carbon dioxide + water
C₆H₁₂O₆ + 6O₂ → 6CO₂ + 6H₂O

Anaerobic respiration

Anaerobic respiration happens without oxygen. In animal cells, such as muscle cells working hard, glucose is broken down to lactic acid. In plant cells and yeast, glucose is broken down to ethanol and carbon dioxide.

glucose → lactic acid
glucose → ethanol + carbon dioxide

Comparing aerobic and anaerobic respirationSpec 8.10

Aerobic and anaerobic respiration compared
Aerobic respirationAnaerobic respiration
Oxygen needed?YesNo
Glucose breakdownComplete breakdownIncomplete breakdown
Energy releasedA large amount per glucose moleculeMuch less per glucose molecule
Products in animalsCarbon dioxide and waterLactic acid
Products in plants and yeastCarbon dioxide and waterEthanol and carbon dioxide
When it happensWhenever enough oxygen is availableWhen oxygen is not available, for example in muscles during vigorous exercise

Aerobic respiration releases much more energy because glucose is completely broken down. Anaerobic respiration releases less energy because glucose is only partly broken down. Both types of respiration are exothermic and both release energy from glucose.

Core practical: the rate of respiration in living organismsSpec 8.11

Respiration can be investigated by measuring how fast a living organism, such as germinating seeds or small invertebrates, uses up oxygen. This is done with a respirometer.

  1. Place the organisms in a boiling tube. Put a layer of soda lime (or another carbon dioxide absorber) in the tube, separated from the organisms by cotton wool or gauze.
  2. Connect the tube to a capillary tube containing a drop of coloured liquid, and seal the apparatus so that no air can get in or out.
  3. Place the tube in a water bath at a set temperature and leave it to settle.
  4. Measure the distance the coloured liquid moves in a set time, for example 5 minutes.
  5. Repeat at different temperatures, and repeat each temperature to calculate a mean.
  6. Set up a control tube with an equal volume of an inert material, such as glass beads, instead of organisms.

The organisms use up oxygen. The carbon dioxide they produce is absorbed, so the volume of gas in the tube falls and the coloured liquid moves towards the organisms. The further it moves in a given time, the faster the rate of respiration.

rate of respiration = distance moved by liquid ÷ time
Variables in the investigation
Type of variableExample
Independent variableTemperature (or the type of organism, if comparing organisms)
Dependent variableDistance moved by the liquid in a set time
Control variablesMass of organisms, volume of soda lime, time, volume of the apparatus, start position of the liquid

A suggested practical for anaerobic respiration is to investigate the effect of glucose concentration on the rate of anaerobic respiration in yeast. The rate can be found by measuring how quickly carbon dioxide is produced, for example by counting bubbles or measuring the volume of gas collected.

Respirometer: a boiling tube in a water bath at a set temperature, with germinating seeds on gauze above soda lime, sealed with a bung connected to a capillary tube containing a drop of coloured liquid beside a scale; an arrow shows the liquid moving towards the organisms.Tap to enlarge
As the organisms use up oxygen, the coloured liquid moves towards them. Soda lime absorbs the carbon dioxide they release.

Quick check

  1. What does exothermic mean?

    Show answer

    Energy is transferred to the surroundings.

  2. Write the word equation for aerobic respiration.

    Show answer

    Glucose + oxygen → carbon dioxide + water.

  3. What are the products of anaerobic respiration in yeast?

    Show answer

    Ethanol and carbon dioxide.

  4. Why does the coloured liquid move towards the organisms in a respirometer?

    Show answer

    The organisms use up oxygen and the carbon dioxide is absorbed, so the volume of gas decreases.