Aseptic techniquesSpec 5.17B
To grow (culture) microorganisms safely and get reliable results, you must stop unwanted microorganisms getting in and stop your culture escaping. This is called aseptic technique.
- Use an autoclave to prepare the sterile growth medium (agar jelly) and the petri dishes. An autoclave sterilises equipment using high-pressure steam, which kills microorganisms already present.
- Use a sterile inoculating loop to transfer microorganisms. The loop is sterilised by heating in a flame until red hot, then cooled before use.
- Keep petri dishes and culture vials covered as much as possible. Only lift the lid briefly when transferring microorganisms.
- After inoculating, fix the lid on with tape and incubate the dish upside down.
- Wash your hands and wipe the bench with disinfectant before and after.