Ventilation and lung volumes

Organisms (Form and function) · Gas exchange · note 3 of 5

Ventilation and lung volumesSpec B3.1.5, B3.1.6

In short

Ventilation is the movement of air into and out of the lungs, caused by pressure changes in the thorax. Contraction of the diaphragm and external intercostal muscles raises thorax volume so pressure falls and air flows in. Tidal volume, vital capacity and the inspiratory and expiratory reserves are measured with a spirometer.

Air always flows from higher to lower pressure. Muscles change the volume of the thorax, which changes the pressure inside it (pressure and volume are inversely related).

Two views of the thorax. Breathing in: external intercostal muscles contract, internal intercostal muscles relax, ribs move up and out, diaphragm contracts and flattens, volume increases and pressure decreases. Breathing out: external intercostal muscles relax, internal intercostal muscles contract, ribs move down and in, diaphragm relaxes and domes upwards, volume decreases and pressure increases. (opens full size in a new tab)
Inspiration and expiration change the volume of the thorax. The breathing-out panel shows forced expiration: in quiet expiration the internal intercostal muscles stay relaxed. The abdominal muscles (not drawn) also contract in forced expiration.
Muscle actions during ventilation
StructureInspiration (breathing in)Expiration (breathing out)
DiaphragmContracts and flattens, moving downRelaxes and is pushed back up into a dome (by elastic recoil and the abdominal organs; in forced expiration also by the abdominal muscles)
External intercostal musclesContract, pulling the ribcage up and outRelax
Internal intercostal musclesRelaxRelaxed in quiet expiration; contract in forced expiration, pulling the ribcage down and in
Abdominal musclesRelax, so abdominal organs can be pushed downRelaxed in quiet expiration; contract in forced expiration, pushing abdominal organs and the diaphragm up
RibsMove up and outMove down and in (by gravity and elastic recoil in quiet expiration; pulled by the internal intercostals in forced expiration)
Thorax volume and pressureVolume increases, pressure falls below atmospheric, air flows inVolume decreases, pressure rises above atmospheric, air flows out

Quiet expiration (breathing out at rest) is mainly passive: the diaphragm and external intercostal muscles relax, and the stretched lungs and chest wall recoil elastically, so thorax volume falls. Forced expiration (during exercise, coughing, or when measuring vital capacity or expiratory reserve) is active: the internal intercostal muscles and the abdominal muscles contract, so more air is pushed out, faster.

Common mistake:

Do not say the internal intercostal muscles contract in every breath out. They contract only in forced expiration; quiet expiration relies on muscle relaxation and elastic recoil.

Lung volumes

Tidal volume
The volume of air breathed in or out in one normal breath.
Inspiratory reserve volume
The extra volume of air that can be breathed in by force after a normal inspiration.
Expiratory reserve volume
The extra volume of air that can be breathed out by force after a normal expiration.
Vital capacity
The largest volume of air that can be breathed out after the deepest possible breath in: tidal volume + inspiratory reserve + expiratory reserve.

Some air always stays in the lungs (the residual volume), so a spirometer cannot measure it and vital capacity is less than total lung volume.

Practical skill:

Measure lung volumes with a digital spirometer, or by breathing out through a tube into an upturned, water-filled, calibrated bottle and reading the volume of water displaced. Repeat each measurement three times and use the mean.

Reading a spirometer trace

On a spirometer trace, a normal breath goes from 2.3 dm³ to 2.8 dm³. The deepest breath in reaches 5.8 dm³ and the deepest breath out falls to 1.2 dm³. Find the tidal volume, inspiratory reserve, expiratory reserve and vital capacity. (Practice data.)

  1. Tidal volume = 2.8 − 2.3 = 0.5 dm³
  2. Inspiratory reserve = 5.8 − 2.8 = 3.0 dm³
  3. Expiratory reserve = 2.3 − 1.2 = 1.1 dm³
  4. Vital capacity = 5.8 − 1.2 = 4.6 dm³ (check: 0.5 + 3.0 + 1.1 = 4.6 dm³)

Answer: TV 0.5 dm³, IRV 3.0 dm³, ERV 1.1 dm³, VC 4.6 dm³

Written and checked against the IB Biology SL specification · Updated October 2026

Frequently asked questions

Why do larger organisms need specialised gas exchange surfaces?

Larger organisms need specialised gas exchange surfaces because their surface area-to-volume ratio is smaller and the distance from their centre to the outside is greater. Diffusion across the body surface alone would be far too slow, so they need large, thin, moist, permeable surfaces such as lungs or gills and a transport system.

How are alveoli adapted for gas exchange?

Alveoli are adapted for gas exchange by their huge total surface area, walls one thin layer of cells thick, a moist lining and a dense capillary network around each one. Surfactant lowers surface tension so alveoli do not collapse, and ventilation with continuous blood flow keeps the concentration gradients steep.

What happens to the diaphragm and intercostal muscles when you breathe in?

When you breathe in, the diaphragm contracts and flattens and the external intercostal muscles contract, pulling the ribs up and out. The volume of the thorax increases, so the pressure inside falls below atmospheric pressure and air flows into the lungs.

All 4 questions on Gas exchange