Speciation, reproductive isolation and differential selection

Ecosystems (Unity and diversity) · Evolution and speciation · note 4 of 4

Speciation, reproductive isolation and differential selectionSpec A4.1.6, A4.1.7

In short

Speciation is the formation of new species by the splitting of a pre-existing species, the only way new species have appeared. A population is divided by reproductive isolation, such as a geographical barrier, and the parts are then subject to differential selection. Their gene pools diverge, as with bonobos and common chimpanzees separated by the Congo River.

Speciation is the formation of new species by the splitting of a pre-existing species. One population divides into two or more groups that evolve separately until they can no longer interbreed to produce fertile offspring. This is the only way in which new species have appeared.

  • Speciation increases the total number of species on Earth. Extinction decreases it.
  • Gradual evolutionary change within a single species over time is not speciation, because the number of species does not increase.

How a species splits

  1. Reproductive isolation: two populations of one species stop interbreeding, so there is no gene flow between them.
  2. Differential selection: the populations live under different conditions, so natural selection favours different alleles in each.
  3. Different mutations also arise in each population, and chance changes in allele frequency happen separately.
  4. Over many generations the gene pools diverge. Eventually the populations could not interbreed and produce fertile offspring even if they met. They are now separate species.

Geographical isolation is one way of achieving reproductive isolation: a physical barrier such as a river, a mountain range or the sea separates the populations.

Bonobos and common chimpanzees

Bonobos (Pan paniscus) and common chimpanzees (Pan troglodytes) are closely related species in the genus Pan. Genetic estimates put the split between the two species at roughly 1 to 2 million years ago. The traditional explanation is that the Congo River formed at about this time and divided the ancestral population. Geological evidence suggests the river is much older, so another hypothesis is that ancestral bonobos crossed to the south bank during a period of unusually low river flow and were then cut off. Either way, the river is the barrier: these apes do not swim, so there is no gene flow across it. Bonobos live only south of the river; common chimpanzees live to the north.

The two populations then faced different selection pressures. One widely discussed explanation is that bonobos, south of the river, had no gorillas competing for ground vegetation, so food was more reliable. This favoured large, tolerant, female-led groups with little aggression. North of the river, chimpanzees share their range with gorillas. This favoured male-dominated groups, more aggression and cooperative hunting.

Simplified map of central Africa with the Congo River arcing between the common chimpanzee (Pan troglodytes) range to the north, shared with gorillas, and the bonobo (Pan paniscus) range to the south, with no gorillas; an inset tree shows an ancestral Pan population splitting into the two species when the river separated them about 1 to 2 million years ago. (opens full size in a new tab)
The Congo River is a geographical barrier: no gene flow, different selection pressures on each bank.
Exam tip:

In speciation answers name both stages: reproductive isolation stops gene flow, and differential selection makes the isolated gene pools diverge. Isolation alone does not create a new species.

Quick check

  1. Define evolution.

    Show answer

    Change in the heritable characteristics of a population.

  2. What do fewer differences in the amino acid sequence of a protein between two species suggest?

    Show answer

    They shared a common ancestor more recently.

  3. Name the five groups of bones in a pentadactyl forelimb.

    Show answer

    Humerus; radius and ulna; carpals; metacarpals; phalanges.

  4. What two processes are needed for speciation?

    Show answer

    Reproductive isolation and differential selection.

Written and checked against the IB Biology SL specification · Updated October 2026

Frequently asked questions

Why is Lamarckism not evolution?

Lamarckism claimed that characteristics acquired during an organism's life are inherited. Acquired changes, such as larger muscles from exercise, do not alter the genes in gametes, so they cannot be passed on. Evolution is change in the heritable characteristics of a population, so only genetic changes that are passed to the next generation count.

How does DNA provide evidence for evolution?

Comparing base sequences of DNA, or amino acid sequences of proteins, shows how related species are. Mutations accumulate in each lineage after it splits, so species with fewer differences shared a common ancestor more recently. These relationships mostly match those found from anatomy, which is independent, strong evidence of common ancestry.

What is the difference between homologous and analogous structures?

Homologous structures share the same basic structure because they were inherited from a common ancestor, but they may have different functions, like the pentadactyl limbs of humans, bats and whales. Analogous structures have the same function but different origins, produced by convergent evolution, like the wings of birds and insects.

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