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Cell biology flashcards

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Cell structure

What are eukaryotic cells?
Cells with genetic material enclosed in a nucleus, plus a cell membrane and cytoplasm. Plant and animal cells are eukaryotic.
What are prokaryotic cells?
Bacterial cells. Much smaller than eukaryotic cells, with cytoplasm and a cell membrane surrounded by a cell wall. Genetic material is not enclosed in a nucleus.
How is the genetic material arranged in a bacterial cell?
As a single DNA loop, not enclosed in a nucleus. There may also be one or more plasmids.
Give the prefixes centi, milli, micro and nano as powers of ten.
Centi 10⁻², milli 10⁻³, micro 10⁻⁶, nano 10⁻⁹.
What is standard form?
A number between 1 and 10 multiplied by a power of ten, for example 3 × 10⁻⁴.
List the parts found in most animal cells.
Nucleus, cytoplasm, cell membrane, mitochondria and ribosomes.
What extra parts do plant cells often have?
Chloroplasts and a permanent vacuole filled with cell sap. Plant and algal cells also have a cell wall made of cellulose.
Function of the nucleus?
Contains the genetic material (DNA) and controls the activities of the cell.
Function of mitochondria?
Where aerobic respiration takes place, releasing energy for the cell.
Function of ribosomes?
Where proteins are made (protein synthesis).
Function of chloroplasts?
They contain chlorophyll and absorb light energy for photosynthesis.
Function of the cell membrane?
Controls the movement of substances into and out of the cell.
Function of the cell wall in plants and algae?
Made of cellulose. It strengthens the cell.
When should you use estimation to compare sub-cellular structures?
When you only need to judge relative size or area, by rounding the measurements to easy numbers.
Required practical 1: which lens do you start with and why?
The lowest-power objective lens, to find the specimen and avoid the lens hitting the slide.
Required practical 1: what must a drawing include?
Clear labels, a title and a magnification scale, drawn in sharp pencil with no shading.
How is a sperm cell adapted for its function?
Tail to swim, many mitochondria for energy, streamlined head, and enzymes in the head to get into the egg.
How is a nerve cell adapted for its function?
It is long to carry impulses over long distances, and has branched connections at the ends to connect with other cells.
How is a muscle cell adapted for its function?
Protein fibres that shorten to contract the cell, and many mitochondria to release energy for contraction.
How is a root hair cell adapted for its function?
Long hair-like projection gives a large surface area to absorb water and mineral ions. Many mitochondria release energy for active transport.
How is a xylem cell adapted for its function?
Forms a hollow tube with no end walls, and strengthened walls, to carry water and mineral ions up the plant.
How is a phloem cell adapted for its function?
Elongated cells joined end to end into tubes. Pores in the end walls let cell sap with dissolved sugars move from cell to cell.
What is cell differentiation?
The process by which cells acquire different sub-cellular structures to carry out a particular function, becoming specialised.
Why is differentiation important?
It lets cells become specialised so they can carry out specific functions efficiently in a multicellular organism.
When do most animal cells differentiate, compared with plant cells?
Most animal cells differentiate at an early stage. Many plant cells keep the ability to differentiate throughout life.
What is cell division mainly used for in mature animals?
Repair and replacement.
Why does an electron microscope show more detail than a light microscope?
It has much higher magnification and resolving power.
What is resolving power?
The ability to distinguish between two points that are close together as separate points.
What is the magnification equation?
Magnification = size of image ÷ size of real object.
How do bacteria reproduce? Triple only
By simple cell division called binary fission, as often as once every 20 minutes with enough nutrients and a suitable temperature.
How can bacteria be grown in the laboratory? Triple only
In a nutrient broth solution, or as colonies on an agar gel plate.
Why must Petri dishes and culture media be sterilised? Triple only
To kill unwanted microorganisms and stop contamination.
Why is an inoculating loop passed through a flame? Triple only
To sterilise it, so only the intended microorganisms are transferred.
Why is the lid of the Petri dish taped on and stored upside down? Triple only
Tape stops microorganisms getting in or out. Upside down stops condensation dripping onto the culture.
Why are school cultures incubated at 25 °C? Triple only
To prevent the growth of pathogens that are harmful to humans.
How do you find the number of bacteria after a given time? Triple only
Divide total time by division time to get the number of divisions, then starting number × 2 to the power of divisions.
Higher tier: how do you give a very large bacterial count in standard form? Triple onlyHigher tier
Write a number between 1 and 10 × a power of ten, for example 16 777 216 = 1.7 × 10⁷ (2 s.f.).
What is a zone of inhibition? Triple only
The clear area around an antiseptic or antibiotic disc where bacteria have not grown. Bigger zone means more effective.
How do you calculate the area of a clear zone? Triple only
Area = πr², where the radius is half the diameter.

Cell division

What are chromosomes?
Structures in the nucleus made of DNA molecules. Each carries a large number of genes.
How are chromosomes found in body cells?
Normally in pairs.
What are the three overall stages of the cell cycle?
Growth and DNA replication; mitosis; division of the cytoplasm and cell membranes.
What happens to the DNA before a cell divides?
It replicates to form two copies of each chromosome.
What happens in mitosis?
One set of chromosomes is pulled to each end of the cell and the nucleus divides.
Why is mitosis important in multicellular organisms?
It is important for growth and development (and repair and replacement).
What is a stem cell?
An undifferentiated cell capable of giving rise to many more cells of the same type, and from which certain other cells can arise from differentiation.
What can stem cells from human embryos do?
They can be cloned and made to differentiate into most different types of human cell.
What can stem cells from adult bone marrow do?
Form many types of cell, including blood cells.
What is meristem tissue?
Tissue in plants whose stem cells can differentiate into any type of plant cell, throughout the life of the plant.
What is therapeutic cloning?
An embryo is produced with the same genes as the patient, so stem cells from it are not rejected by the patient's body.
Give two risks or objections to using stem cells.
Risk of transfer of viral infection; some people have ethical or religious objections.
How can plant stem cells be used?
To clone rare species to protect them from extinction, and to clone crop plants with desirable features such as disease resistance.

Transport in cells

Define diffusion.
The spreading out of the particles of any substance in solution, or particles of a gas, resulting in a net movement from an area of higher concentration to an area of lower concentration.
Give three substances that move by diffusion in the body.
Oxygen and carbon dioxide in gas exchange, and urea from cells into blood plasma.
Name three factors that affect the rate of diffusion.
Concentration gradient, temperature and surface area of the membrane.
Why does a single-celled organism not need an exchange surface?
It has a large surface area to volume ratio, so enough molecules can diffuse in and out.
How do you calculate surface area to volume ratio?
Surface area ÷ volume. For a cube: surface area = 6 × side², volume = side³.
Why do large multicellular organisms need exchange surfaces and a transport system?
Their surface area to volume ratio is small, so diffusion across the outside would not supply all cells.
What makes an exchange surface more effective?
Large surface area, thin membrane (short diffusion path), efficient blood supply (animals) and ventilation (animal gas exchange).
How is the small intestine adapted for exchange?
Villi give a large surface area, a thin wall gives a short diffusion path, and a good blood supply carries absorbed molecules away.
How are the lungs adapted for gas exchange?
Many alveoli give a large surface area, thin walls, a good blood supply and ventilation.
How are the gills of a fish adapted for exchange?
Many gill filaments with lamellae give a large surface area, thin, with a good blood supply and water flowing over them.
How are roots and leaves adapted for exchange?
Root hairs give a large surface area for water and ions. Leaves are flat and thin with air spaces and stomata for gas exchange.
Define osmosis.
The diffusion of water from a dilute solution to a concentrated solution through a partially permeable membrane.
How do you calculate percentage change in mass?
(final mass − initial mass) ÷ initial mass × 100.
Osmosis required practical (RP3 Triple, RP2 Combined): what is measured and what is varied?
Measure the mass of plant tissue before and after. Vary the concentration of salt or sugar solution.
Osmosis required practical: what does a loss in mass show?
Water moved out of the cells by osmosis because the solution was more concentrated than the cell contents.
Define active transport.
Movement of substances from a more dilute to a more concentrated solution, against a concentration gradient, using energy from respiration.
Give two examples of active transport.
Mineral ions into plant root hairs from the soil, and sugar from the gut into the blood.
How does active transport differ from diffusion?
It moves substances against the concentration gradient and needs energy from respiration.